Journal: International Journal of Molecular Medicine
Article Title: Platelet activation stimulates macrophages to enhance ulcerative colitis through PF4/CXCR3 signaling
doi: 10.3892/ijmm.2025.5519
Figure Lengend Snippet: Platelet or C-X-C motif chemokine receptor 3 inhibition may prevent UC in mice. (A) Flow chart for modeling of UC in Balb/c mice; after 3 days of pretreatment with clopidogrel or AMG487, mice were given 2.5% DSS solution freely. The DSS solution was changed every 2 days and the mice were sacrificed on the last day of modeling. (B) Body weight of mice during DSS modeling was recorded daily. (C) Daily disease activity index score of mice during modeling, which was calculated according to changes in body weight, stool softness and fecal occult blood. (D) After modeling, the colon length of mice in each group was recorded and compared (n=5). (E) At the end of modeling, the colons were collected and pathological sections were made, and the pathological scores were calculated according to the degree of inflammatory cell infiltration, degree of crypt abnormality and degree of mucosal injury measured by hematoxylin and eosin staining. Scale bar, 200 μ m. Continuous data are presented as the mean ± SD, whereas categorical data are presented as the median (range) (n=5). * P<0.05, ** P<0.01 and *** P<0.001. UC, ulcerative colitis; DSS, dextran sulfate sodium.
Article Snippet: The clopidogrel group was administered an oral gavage of 20 mg/kg/day clopidogrel (MedChemExpress) ( , ), whereas the AMG487 group was administered an intraperitoneal injection of 5 mg/kg/day AMG487 ( ).
Techniques: Inhibition, Activity Assay, Staining
